Related Experiment Videos
New approaches to peptide synthesis with the help of trypsin
Mitin YuV1, N P Zapevalova, Gorbunova EYu
1Institute of Protein Research, Academy of Sciences, Puschino, Moscow Region, USSR.
Biomedica Biochimica Acta
|January 1, 1991
Abstract:
Picolyl esters of amino acids are suitable inverse substrates for trypsin. These esters can be used for peptide synthesis catalyzed by trypsin. Study of hydrolysis of non-specific substrates for trypsin permits to conclude, that hydrogen band in P'2 subsite is very important for their reactivity. Carboxamido methyl esters (CAM-esters) are the most suitable substrates for trypsin catalyzed peptide synthesis. Using CAM-esters and trypsin several di-,tri- and tetra-peptides were synthesized with good yields.