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Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
Published on: April 30, 2010
The application of differential display as a gene profiling tool
Ken Chien-Neng Chang1, Barry Komm, Nichole Bayer Arnold
1Osteoporosis Research, Women's Health Research Institute, Wyeth Research, Collegeville, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 26, 2008
Summary
Differential display is a powerful gene expression profiling technique. This review covers its evolution, a new protocol, and its continued relevance in cancer research.
Area of Science:
- Molecular Biology
- Genomics
- Biotechnology
Background:
- Differential display (DD) is a gene expression profiling technique.
- Established in 1992, DD identifies differences in gene expression between samples.
- Numerous modifications have enhanced DD's utility over time.
Purpose of the Study:
- To review the evolution and applications of differential display.
- To present a reproducible, semi-high-throughput DD protocol.
- To compare DD with other expression profiling technologies.
Main Methods:
- Review of historical and modified differential display techniques.
- Description of a novel, reproducible semi-high-throughput DD protocol.
- Case study: Application of DD in pancreatic cancer cell analysis.
Main Results:
- The described DD protocol demonstrated high reproducibility.
- Successful application of DD in identifying differentially expressed genes in pancreatic cancer.
- Review highlights diverse successful applications of DD across various research scenarios.
Conclusions:
- Differential display remains a valuable and applicable gene expression profiling tool.
- The presented protocol offers a robust method for DD analysis.
- DD continues to be a relevant technique for molecular research, including cancer studies.
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