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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
Published on: July 5, 2016
Digital holographic microscopy for live cell applications and technical inspection
1Laboratory of Biophysics, University of Muenster, Robert-Koch-Strasse 45, D-48129 Muenster, Germany.
Applied Optics
|February 2, 2008
Summary
Digital holographic microscopy offers quantitative phase contrast imaging for reflective surfaces and marker-free live cell analysis. Its numerical focus adjustment enables multifocus imaging for diverse applications.
Area of Science:
- Biomedical optics
- Metrology
- Microscopy
Background:
- Digital holographic microscopy (DHM) provides quantitative phase contrast imaging.
- It is applicable to both reflective surfaces and transparent specimens like living cells.
- Marker-free analysis of biological samples is a key advantage.
Purpose of the Study:
- To provide an overview of digital holographic microscopy methods.
- To demonstrate the applications of DHM in various fields.
- To highlight the capabilities of DHM for multifocus imaging.
Main Methods:
- Quantitative phase contrast metrology using digital holography.
- Numerical focus adjustment for multifocus imaging.
- Holographic data processing for image reconstruction.
Main Results:
- Demonstrated DHM for analyzing engineered surfaces.
- Showcased DHM for marker-free investigation of living cells.
- Validated multifocus imaging capabilities through numerical refocusing.
Conclusions:
- Digital holographic microscopy is a versatile technique for quantitative phase contrast imaging.
- DHM facilitates advanced applications including multifocus imaging and live cell analysis.
- The technology is suitable for both biological and materials science investigations.
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