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Related Concept Videos

Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Updated: Jul 7, 2026

Trypsinizing and Subculturing Mammalian Cells
05:59

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

Techniques for mammalian cell tissue culture.

Mary C Phelan1

  • 1Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary

This guide details sterile techniques and cell culture methods, including subculturing, freezing, thawing, and counting cells. It provides essential protocols for preparing cells for transport and maintaining cell viability.

Area of Science:

  • Cell Biology
  • Microbiology
  • Biotechnology

Background:

  • Maintaining cell line integrity is crucial for reproducible research.
  • Standardized protocols ensure consistency in cell culture experiments.

Purpose of the Study:

  • To provide comprehensive protocols for essential cell culture techniques.
  • To guide researchers in sterile handling and cell preparation.

Main Methods:

  • Detailed sterile technique principles.
  • Preparation of culture media.
  • Trypsinizing and subculturing monolayer cultures.
  • Passaging suspension cultures.
  • Cell freezing and thawing.
  • Cell counting using a hemacytometer.

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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

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Last Updated: Jul 7, 2026

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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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  • Preparing cells for transport.

    • Main Results:

    • Established protocols for various cell culture manipulations.
    • Demonstrated methods for cell viability assessment.
    • Outlined procedures for safe cell preservation and shipment.

    Conclusions:

    • Adherence to sterile techniques is paramount for successful cell culture.
    • These protocols facilitate reliable cell propagation and experimental setup.
    • Effective cell preparation ensures successful research outcomes.