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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: Jul 5, 2026

Trypsinizing and Subculturing Mammalian Cells

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

Techniques for mammalian cell tissue culture.

Mary C Phelan¹

¹Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.

Current Protocols in Neuroscience

|April 23, 2008

Summary

This guide details sterile techniques and cell culture methods, including media preparation, cell passaging, freezing, thawing, and counting. It provides essential protocols for handling various cell types and preparing them for transport.

Area of Science:

Cell Biology
Microbiology

Background:

Maintaining aseptic conditions is crucial for successful cell culture.
Standardized protocols ensure reproducibility in cell-based research.

Purpose of the Study:

To provide comprehensive protocols for essential cell culture techniques.
To guide researchers in sterile technique and cell handling.

Main Methods:

Detailed explanation of sterile technique principles.
Step-by-step protocols for preparing culture media.
Methods for trypsinizing, subculturing, and passaging cells (monolayer and suspension).
Procedures for cell cryopreservation (freezing and thawing).
Hemacytometer-based cell counting techniques.
Protocols for preparing cells for shipment.

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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

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Trypsinizing and Subculturing Mammalian Cells

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Published on: June 12, 2008

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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

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Main Results:

Established protocols for diverse cell culture manipulations.
Demonstrated methods for maintaining cell viability during handling and storage.
Provided guidelines for accurate cell quantification.

Conclusions:

Mastery of these techniques is fundamental for reliable cell culture.
The described methods support efficient cell line maintenance and experimental preparation.