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Related Concept Videos

Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
Plant Tissue Culture02:57

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Trypsinizing and Subculturing Mammalian Cells
05:59

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

Techniques for mammalian cell tissue culture.

Mary C Phelan1

  • 1Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.

Current Protocols in Toxicology
|October 26, 2010
PubMed
Summary

This guide details sterile techniques and cell culture methods, including subculturing, freezing, and counting cells. It provides essential protocols for researchers working with cell cultures and preparing them for transport.

Area of Science:

  • Cell Biology
  • Microbiology

Background:

  • Cell culture is fundamental to biological research.
  • Maintaining aseptic conditions and proper cell handling are critical for experimental success.

Purpose of the Study:

  • To provide comprehensive protocols for essential cell culture techniques.
  • To guide researchers in sterile technique and cell preparation.

Main Methods:

  • Detailed discussion of sterile technique principles.
  • Step-by-step protocols for media preparation.
  • Instructions for trypsinizing, subculturing, passaging, freezing, thawing, and counting cells.
  • Methods for preparing cells for transport.

Main Results:

  • Established protocols for various cell culture manipulations.

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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Last Updated: Jun 7, 2026

Trypsinizing and Subculturing Mammalian Cells
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  • Demonstrated methods for accurate cell counting.
  • Guidelines for preserving cell viability through freezing and thawing.

    • Conclusions:

    • Adherence to sterile techniques ensures reliable cell culture outcomes.
    • Properly executed protocols are vital for cell viability and experimental integrity.
    • This appendix serves as a practical resource for cell culture laboratories.