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A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
Published on: October 17, 2016
Biocompatibility and biodegradation of polyester and polyfumarate based-scaffolds for bone tissue engineering
M Susana Cortizo1, M Silvina Molinuevo, Ana M Cortizo
1Instituto de Investigaciones Fisicoquímica Teóricas y Aplicadas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900 La Plata, Argentina. cortizo@biol.unlp.edu.ar
Journal of Tissue Engineering and Regenerative Medicine
|February 15, 2008
Summary
New biodegradable polymeric scaffolds support bone regeneration. Polyesters and polyfumarates were tested for osteoblast support, showing promising results for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Chemistry
Background:
- Biodegradable and biocompatible polymeric scaffolds are crucial for tissue regeneration.
- Developing effective scaffolds for bone regeneration remains a key challenge in regenerative medicine.
Purpose of the Study:
- To develop and characterize polymeric scaffolds for bone regeneration.
- To evaluate the biocompatibility and osteogenic potential of selected polyesters and polyfumarates.
Main Methods:
- Synthesis and characterization of poly-beta-propiolactone (PBPL), poly-epsilon-caprolactone (PCPL), polydiisopropyl fumarate (PDIPF), and polydicyclohexyl fumarate (PDCF) films.
- Surface analysis using scanning electron microscopy and water contact angle measurements.
- In vitro degradation studies in PBS and with macrophages.
- Biocompatibility and cytotoxicity assays using MC3T3E1 and UMR106 osteoblastic cell lines.
Main Results:
- Polyesters exhibited rougher, more porous surfaces than polyfumarates.
- Macrophages actively degraded all polymeric films; only PBPL showed hydrolytic degradation.
- Osteoblasts adhered, grew, and showed no cytotoxicity on all scaffolds.
- UMR106 cell proliferation was significantly higher on polyester scaffolds (PBPL, PCPL).
- UMR106 cells displayed increased alkaline phosphatase activity, indicating osteogenic differentiation.
Conclusions:
- The developed synthetic polymeric scaffolds support osteoblast adhesion, growth, and differentiation.
- These materials show potential for applications in bone tissue regeneration.
- Material properties, such as surface characteristics and degradation behavior, influence cell response.
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