Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue sample collection for proteomics analysis.

Jose I Diaz1, Lisa H Cazares, O John Semmes

  • 1Cancer Therapy Research Center's Institute for Drug Development, University of Texas Health Science Center, San Antonio, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phosphorylation of SNAP-25 at Ser187 is enhanced following its cleavage by Botulinum Neurotoxin Serotype A, promoting the dominant-negative effect of the resulting fragment.

PLoS pathogens·2025
Same author

Ancestry-Dependent Immunologic and Prognostic Effects Characterize the Prostate Cancer Urinary Proteome.

bioRxiv : the preprint server for biology·2025
Same author

Characterization of prostate macrophage heterogeneity, foam cell markers, and CXCL17 upregulation in a mouse model of steroid hormone imbalance.

Scientific reports·2024
Same author

Small extracellular vesicles: Roles and clinical application in prostate cancer.

Advances in cancer research·2024
Same author

Prostate cancer reshapes the secreted and extracellular vesicle urinary proteomes.

Nature communications·2024
Same author

PROSTATE CELL HETEROGENEITY AND CXCL17 UPREGULATION IN MOUSE STEROID HORMONE IMBALANCE.

bioRxiv : the preprint server for biology·2024
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study details optimal tissue collection for proteomic analysis, emphasizing immediate freezing, storage, and microdissection. Pathologist collaboration and laser capture microscopy are key for accurate molecular findings from tissue targets.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pathology

Background:

  • Accurate proteomic analysis relies on high-quality tissue sample collection.
  • Standardized procedures are crucial for preserving protein integrity in tissue specimens.
  • The role of pathologists in molecular diagnostics is increasingly important.

Purpose of the Study:

  • To outline a detailed protocol for tissue specimen collection for proteomic analysis.
  • To highlight critical steps including timing, freezing, storage, and microdissection.
  • To emphasize the collaborative role of pathologists in molecular studies.

Main Methods:

  • Immediate freezing and proper storage of tissue samples.
  • Microdissection techniques for isolating specific cellular components ('tissue targets').

Related Experiment Videos

  • Application of techniques like Surface-Enhanced Laser Desorption/Ionization (SELDI), Matrix-Assisted Laser Desorption/Ionization (MALDI), and 2D Gel Electrophoresis (2DGE).

    • Main Results:

    • A standardized procedure for tissue collection ensures sample integrity for proteomic analysis.
    • Pathologist expertise is vital for correlating morphological findings with molecular data.
    • Laser capture microscopy enhances the efficiency of isolating specific tissue targets.

    Conclusions:

    • Effective tissue collection protocols are essential for successful proteomic studies.
    • The integration of pathology expertise and advanced tools like laser capture microscopy improves molecular analysis.
    • Standardized sample handling maximizes the utility of tissue banks for research.