Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spliceosome assembly and composition.

Arianne J Matlin1, Melissa J Moore

  • 1Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.

Advances in Experimental Medicine and Biology
|April 3, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

mRNA COVID-19 vaccines: science versus misinformation.

RNA (New York, N.Y.)·2026
Same author

Attenuating ribosome load improves protein output from mRNA by limiting translation-dependent mRNA decay.

Cell reports·2024
Same author

Translation-dependent and -independent mRNA decay occur through mutually exclusive pathways defined by ribosome density during T cell activation.

Genome research·2024
Same author

Tailor made: the art of therapeutic mRNA design.

Nature reviews. Drug discovery·2023
Same author

An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts.

Nature biotechnology·2022
Same author

Chemical optimization of siRNA for safe and efficient silencing of placental sFLT1.

Molecular therapy. Nucleic acids·2022
Same journal

Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases.

Advances in experimental medicine and biology·2026
Same journal

Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.

Advances in experimental medicine and biology·2026
Same journal

The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

Advances in experimental medicine and biology·2026
Same journal

Regulation of the Anti-termination RNA Transcription Complex by Lon-Mediated Lambda N Degradation.

Advances in experimental medicine and biology·2026
Same journal

PCNA Macromolecular Complexes: PCNA Serves as a Molecular Hub Regulating Multiple Cellular Processes Inside and Outside of the Nucleus.

Advances in experimental medicine and biology·2026
Same journal

Dynamic Assemblies in Genome Maintenance.

Advances in experimental medicine and biology·2026
See all related articles

Cells regulate alternative splicing by controlling spliceosome assembly. Understanding these mechanisms is key to explaining splice site selection in mammals and improving splicing fidelity through proofreading.

Area of Science:

  • Molecular biology
  • Cellular biology
  • Genetics

Background:

  • Alternative splicing is a key process for generating protein diversity.
  • Spliceosome assembly dynamics dictate alternative splice site usage.
  • Understanding spliceosome regulation is crucial for deciphering gene expression control.

Purpose of the Study:

  • To review spliceosome assembly mechanisms in mammalian cells.
  • To highlight regulatory factors and steps in alternative splice site selection.
  • To discuss in vivo versus in vitro assembly pathways and proofreading.

Main Methods:

  • Literature review of spliceosome assembly studies.
  • Analysis of regulatory factors in alternative splicing.
  • Comparison of in vivo and in vitro spliceosome assembly.

Related Experiment Videos

Main Results:

  • Spliceosome assembly is a regulated process influencing alternative splicing.
  • Specific factors and steps are targeted for regulation during splice site selection.
  • Differences and similarities exist between in vivo and in vitro assembly pathways.

Conclusions:

  • A thorough understanding of spliceosome assembly is essential for alternative splicing.
  • Regulatory mechanisms governing spliceosome assembly are critical for fidelity.
  • Further research into in vivo assembly and proofreading will advance the field.