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Updated: Jul 5, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Label-free cell-based assay using localized surface plasmon resonance biosensor.

Tatsuro Endo1, Shohei Yamamura, Kagan Kerman

  • 1Department of Mechano-Micro Engineering, Interdisciplinary Graduate of School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan. endo.t.ab@m.titech.ac.jp

Analytica Chimica Acta
|April 19, 2008
PubMed
Summary

This study introduces a novel label-free biosensor using localized surface plasmon resonance (LSPR) for detecting cell metabolites. The developed biosensor offers a low-cost, sensitive platform for analyzing biomolecular interactions in biological samples.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Biosensing

Background:

  • Cellular analysis is crucial for understanding life activities, impacting biology and biotechnology.
  • Existing methods for cell analysis can be complex and costly.

Purpose of the Study:

  • To develop a novel, label-free cell-based assay for detecting cell metabolites.
  • To utilize a localized surface plasmon resonance (LSPR) biosensor with a core-shell nanoparticle substrate.

Main Methods:

  • Fabrication of an LSPR biosensor using a core-shell nanoparticle layer.
  • Immobilization of antibodies onto the LSPR biosensor surface.
  • Detection of refractive index changes caused by antigen-antibody interactions with cell metabolites from mouse thymus cells.

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Last Updated: Jul 5, 2026

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Main Results:

  • Achieved a detection limit of 10 pg/mL for cell metabolites.
  • Successfully performed time-course analysis of cell metabolism.
  • Demonstrated the biosensor's capability in detecting specific antibody-metabolite reactions.

Conclusions:

  • The LSPR-based biosensor is a promising, low-cost platform for detecting biomolecular interactions.
  • Offers advantages like simplified experimental setup and low sample volume requirements.
  • Enables sensitive and efficient analysis of cell metabolites and metabolism.