Related Experiment Video
Updated: Jan 31, 2026

Author Spotlight: Advancing Coral Culture - Creating a Semi-Quantitatively Controlled Microenvironment System to Counter Current Limitations
Published on: July 21, 2023
Monitoring cell growth
1National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
Abstract:
This appendix provides two protocols for monitoring cell growth. Counting cells using a hemacytometer is tedious but it allows one to effectively distinguish live cells from dead cells (using Trypan Blue exclusion). In addition, this procedure is less subject to errors due to cell clumping or heterogeneity of cell size. The use of an electronic cell counter is quicker and easier than counting cells using a hemacytometer. However, an electronic cell counter as currently constructed does not distinguish live from dead cells in a reliable fashion and is subject to error due to the presence of cell clumps. Overall, the electronic cell counter is best reserved for repetitive and rapid counting of fresh peripheral blood cells and should be used with caution when counting cell populations derived from tissues.
Related Concept Videos
Holter Monitor: 24-Hour Monitoring
Cells Coordinate Growth and Proliferation
Population Growth
Meristems and Plant Growth
Primary and Secondary Growth in Roots and Shoots
Self-Presentation: Self-Monitoring and Self-Handicapping

