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Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Jul 5, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

Agarose gel electrophoresis.

Daniel Voytas1

  • 1Iowa State University, Ames, Iowa.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary

Agarose gel electrophoresis effectively separates DNA fragments ranging from 0.5 to 25 kilobases (kb). This method involves preparing the gel, running the DNA samples, and visualizing the results using staining or UV light.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Agarose gel electrophoresis is a fundamental technique in molecular biology.
  • It is widely used for DNA fragment analysis and purification.
  • Accurate separation depends on agarose concentration and running conditions.

Purpose of the Study:

  • To provide a comprehensive protocol for agarose gel electrophoresis.
  • To detail the steps for separating, identifying, and purifying DNA fragments.
  • To offer guidance on gel preparation, sample loading, and visualization.

Main Methods:

  • Preparation of agarose gels with appropriate concentrations.
  • Electrophoresis of DNA samples at optimized voltage and time.
  • Staining or direct visualization of DNA using UV light and ethidium bromide.

More Related Videos

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
09:24

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Published on: June 14, 2016

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
07:20

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

Published on: August 1, 2025

Related Experiment Videos

Last Updated: Jul 5, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
09:24

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Published on: June 14, 2016

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
07:20

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

Published on: August 1, 2025

Main Results:

  • Successful separation of DNA fragments within the 0.5- to 25-kb size range.
  • Identification and purification of specific DNA fragments.
  • Demonstration of visualization techniques including staining and UV illumination.

Conclusions:

  • Agarose gel electrophoresis is a robust and versatile method for DNA analysis.
  • The protocol ensures reliable separation and purification of DNA fragments.
  • Recommendations for gel photography enhance data documentation.