Isolation and purification of C3 from human plasma
Lynda D O'Rear1, Gordon D Ross1
1University of Louisville, Louisville, Kentucky.
Current Protocols in Immunology
|April 25, 2008
Abstract:
The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.
