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Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates
05:53

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Published on: July 6, 2013

A mouse model for cytomegalovirus infection

W Brune1, H Hengel, U H Koszinowski

  • 1University of Munich, Max von Pettenkofer Institute, Munich, Germany.

Current Protocols in Immunology
|April 25, 2008
PubMed

Insights

This study details methods for infecting mice with murine cytomegalovirus (mCMV). It includes protocols for virus propagation, preparing virulent strains, and quantifying viral load using plaque-forming cell assays.

Area of Science:

  • Virology
  • Immunology
  • Animal Models

Background:

  • Murine cytomegalovirus (mCMV) is a significant pathogen in research settings.
  • Understanding mCMV infection dynamics is crucial for developing effective countermeasures.
  • Standardized protocols are needed for consistent mCMV research.

Purpose of the Study:

  • To describe standardized procedures for infecting mice with mCMV.
  • To provide methods for mCMV propagation and preparation of virulent strains.
  • To detail a plaque-forming cell (PFC) assay for viral quantification.

Main Methods:

  • Infection of newborn and adult mice with mCMV.
  • Propagation of mCMV in cell cultures.
  • Preparation of virulent mCMV from infected mouse salivary glands.
  • Murine embryonic fibroblast preparation.
  • Plaque-forming cell (PFC) assay for mCMV titration.

Main Results:

  • Established protocols for successful mCMV infection in mice.
  • Demonstrated methods for generating high-titer mCMV stocks.
  • Validated the PFC assay for accurate measurement of mCMV titers.

Conclusions:

  • The described procedures provide a robust framework for mCMV research.
  • Standardized methods facilitate reproducible studies on mCMV pathogenesis and immunity.
  • Accurate viral quantification is essential for evaluating antiviral strategies.

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