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Related Experiment Video

Updated: Feb 28, 2026

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Isolation of mouse mononuclear cells.

A M Kruisbeek¹

¹Netherlands Cancer Institute, Amsterdam, The Netherlands.

Current Protocols in Immunology

|April 25, 2008

Summary

This summary is machine-generated.

This unit details methods for preparing mouse lymphocyte cell suspensions. It covers removing red blood cells and dead cells, essential for subsequent functional response assays.

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Last Updated: Feb 28, 2026

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

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Published on: February 16, 2018

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

Published on: December 27, 2014

Area of Science:

Immunology
Cell Biology

Background:

Accurate lymphocyte analysis requires pure, viable cell populations.
Standard laboratory protocols often involve complex cell preparation steps.

Purpose of the Study:

To describe essential techniques for preparing lymphoid cell suspensions.
To outline methods for eliminating contaminating red blood cells and non-viable cells.

Main Methods:

Preparation of single-cell suspensions from lymphoid tissues.
Red blood cell lysis techniques.
Viability assessment and dead cell removal strategies.

Main Results:

Obtained pure lymphocyte suspensions free from erythrocytes and debris.
Demonstrated efficient removal of dead cells, enhancing sample quality.

Conclusions:

These fundamental cell preparation techniques are critical for reliable downstream functional assays.
Optimized lymphocyte isolation ensures accurate measurement of immune cell responses.