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Related Experiment Video

Updated: Jul 5, 2026

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Published on: March 1, 2011

Measurement of interleukin 6.

R P Nordan¹, C D Richards, J Gauldie

¹National Cancer Institute, Bethesda, Maryland, USA.

Current Protocols in Immunology

|April 25, 2008

Summary

This summary is machine-generated.

This study presents a sensitive bioassay for quantifying Interleukin 6 (IL-6) across multiple species by measuring cell proliferation. This method offers a viable alternative to ELISA kits for assessing IL-6 bioactivity.

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Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

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Last Updated: Jul 5, 2026

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Published on: March 1, 2011

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

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Published on: March 8, 2024

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

Area of Science:

Immunology
Cell Biology

Background:

Interleukin 6 (IL-6) is a key cytokine involved in immune responses and cellular processes.
IL-6 is produced by various cells upon stimulation, highlighting its broad biological significance.

Purpose of the Study:

To describe a simple and sensitive bioassay for quantifying Interleukin 6 (IL-6) in multiple species.
To provide protocols for maintaining cell lines and preparing reagents for IL-6 measurement.

Main Methods:

Utilized an IL-6-dependent murine B cell hybridoma cell line (B9) to assess IL-6 bioactivity.
Developed protocols for B9 cell maintenance, IL-6 standard preparation, and supernatant collection.

Main Results:

Established a sensitive assay for human, rat, rabbit, pig, and mouse IL-6.
Demonstrated the utility of the B9 cell proliferation assay for IL-6 quantification.

Conclusions:

The described bioassay offers a sensitive and species-versatile method for measuring IL-6 bioactivity.
ELISA kits are an alternative for human/mouse IL-6 measurement but do not assess bioactivity.