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Isolation and generation of human dendritic cells
Thomas F Tedder1, Paul J Jansen1
1Duke University Medical Center, Durham, North Carolina.
Current Protocols in Immunology
|April 25, 2008
Summary
This study details methods to isolate and generate dendritic cells (DCs), specialized antigen-presenting cells, from human blood. Researchers can enrich DC populations or generate them from monocytes for various immunological studies.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DCs) are critical antigen-presenting cells (APCs) for initiating immune responses.
- Mature DCs typically constitute a small fraction (0.2%) of human blood mononuclear cells.
Purpose of the Study:
- To describe methods for isolating and generating dendritic cells from human blood.
- To provide techniques for enriching DC populations and depleting them for comparative studies.
Main Methods:
- Cell enrichment techniques to increase DC frequency from human blood mononuclear cells.
- Fluorescence-activated cell sorting for highly purified DC preparations.
- Cytokine-driven in vitro generation of DCs from monocytes.
- Negative selection for DC-depleted cell preparations.
Main Results:
- Established methods for significantly increasing the yield of dendritic cells.
- Developed protocols for generating purified dendritic cell populations.
- Provided a negative selection approach for creating control cell populations.
Conclusions:
- The described methods facilitate the isolation and generation of dendritic cells for research.
- These techniques support various immunological studies requiring enriched or depleted DC populations.
