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Iodination of soluble and membrane-bound proteins

L E Samelson1

  • 1National Institute of Child Health and Human Development, Bethesda, Maryland.

Current Protocols in Immunology
|April 25, 2008
PubMed

Coupling of radioactive (125)I to soluble proteins is a critical step in a number of immunoassays. Chloramine T-catalyzed iodination of soluble proteins, such as immunoglobulins, is described in this unit. Protocols using lactoperoxidase-catalyzed iodination of soluble or membrane-bound proteins are also described.

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