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Updated: Jul 4, 2026
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
Published on: April 10, 2018
Development of a more efficient algorithm for identifying false-positive reactivity results in a dengue virus
Harry E Prince1, Cindy Yeh, Mary Lapé-Nixon
1Focus Diagnostics Inc., Cypress, California 90630, USA. hprince@focusdx.com
Abstract:
Of 2,692 sera screened for dengue virus immunoglobulin M by using a mu-capture enzyme-linked immunosorbent assay (ELISA), 954 had equivocal (index from 0.90 to 1.10) or positive (index of >1.10) results and were retested using a background subtraction (BS) ELISA that identifies screen false positives. No false positives were found among 427 sera with screen ELISA indices of >6.00; thus, retesting this specimen subset by BS ELISA is unnecessary.
