Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid, single-step nucleic acid detection.

Kyle A Cissell1, Sean Campbell, Sapna K Deo

  • 1Department of Chemistry and Chemical Biology, Indiana University Purdue University Indianapolis, 402 N. Blackford St., Room LD 326, Indianapolis, IN 46202, USA.

Analytical and Bioanalytical Chemistry
|June 20, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting emerging respiratory pathogens with clamp peptides: Broad-spectrum inhibition of viral entry in 3D human lung models.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Vaginal polycyclic aromatic hydrocarbons (PAHs), HPV infection, and vaginal microbiome in firefighters: A cross-sectional study.

Journal of hazardous materials·2026
Same author

Quorum-Sensing Molecules Are Elevated in Long-Standing Ulcerative Colitis and Are Linked to the Development of Colitis-Associated Cancer.

Gastroenterology·2026
Same author

Negative regulation of T<sub>H</sub>17-mediated inflammation by the nuclear receptor REV-ERBβ.

bioRxiv : the preprint server for biology·2026
Same author

DISTRIBUTED DELAY STABILIZES BISTABLE GENETIC NETWORKS.

ArXiv·2026
Same author

Arteriovenous Malformations (AVMs): Molecular Pathogenesis, Clinical Features, and Emerging Therapeutic Strategies.

Biomolecules·2025
Same journal

Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.

Analytical and bioanalytical chemistry·2026
Same journal

Advancing total uronic acid quantification using a stable isotope dilution approach: validation and application to plant- and algal-derived polysaccharides.

Analytical and bioanalytical chemistry·2026
Same journal

Electroanalytical method development for the receptor tyrosine kinase inhibitor lenvatinib using a Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>-MXene based molecularly imprinted polymer modified carbon electrode.

Analytical and bioanalytical chemistry·2026
Same journal

Impact of blood contamination on hydrophilic metabolomics in human meningioma tissue.

Analytical and bioanalytical chemistry·2026
Same journal

A UiO-66@MR paper-based colorimetric sensor for sensitive detection of food spoilage volatile organic compounds and visual freshness monitoring.

Analytical and bioanalytical chemistry·2026
Same journal

An electrochemical sensing platform based on UiO@TATF COF/CB composites for the detection of nitrofurazone.

Analytical and bioanalytical chemistry·2026
See all related articles

Researchers developed a rapid nucleic acid detection method using bioluminescence resonance energy transfer (BRET) and quantum dots. This assay offers sensitive, homogeneous detection in under 30 minutes, improving upon traditional blotting techniques.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Traditional nucleic acid detection methods are often time-consuming and require multiple steps.
  • There is a need for rapid, sensitive, and homogeneous assays for nucleic acid detection.

Purpose of the Study:

  • To develop a novel, rapid, and sensitive method for nucleic acid detection.
  • To utilize bioluminescence resonance energy transfer (BRET) with quantum dots as a detection platform.

Main Methods:

  • Developed a BRET-based assay using Renilla luciferase as a donor and quantum dots as an acceptor.
  • Employed a competitive assay format correlating BRET signal with target nucleic acid quantity.
  • Validated the assay in both in vitro buffer and cellular matrices.

Related Experiment Videos

Main Results:

  • Achieved detection of as little as 4 pmol (20 nM) of nucleic acid.
  • Demonstrated a homogeneous, single-step assay format.
  • Completed detection in as little as 30 minutes, significantly faster than conventional methods.
  • This is the first report using quantum dots as BRET acceptors for nucleic acid hybridization assays.

Conclusions:

  • The developed BRET-based method provides a rapid, sensitive, and homogeneous approach for nucleic acid detection.
  • Quantum dots serve as effective BRET acceptors in nucleic acid hybridization assays.
  • This technique offers a significant advancement over existing time-consuming methods.