Related Experiment Video
Updated: Jul 24, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
Published on: July 22, 2013
[Effect of thiol-oxidizing agents on several enzymes in rat liver mitochondria]
Abstract:
The sulphydryl groups of nonprotein thiols and proteins of mitochondria, activity of enzymes, oxidative phosphorylation and swelling of these organellas were studied as affected by the thiol-oxidizing agents. Diamide and azoester are shown to oxidize SH-groups of mitochondria, inhibit isocytrate dehydrogenase and succinate: cytochrome oxidation and inhibits energy-dependent swelling of mitochondria. Under the effect of azoester the rate of oxygen consumption by mitochondria increases and there occurs their sharp swelling.
More Related Videos
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
08:03Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
Published on: March 7, 2025
Related Concept Videos
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
Preparation and Reactions of Thiols
Electron Transport Chain: Complex III and IV
Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Bioactivation and Tissue Toxicity