Related Experiment Video
Updated: Jul 3, 2026

09:20
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
Published on: August 11, 2020
Development of a stage-scanning system for high-resolution confocal STEM
Journal of Electron Microscopy
|July 8, 2008
Summary
A new stage-scanning system allows for precise, three-dimensional movement of specimens in transmission electron microscopy. This innovation enables high-resolution confocal scanning transmission electron microscopy (STEM) imaging with a stable electron-optics setup.
Area of Science:
- Materials Science
- Microscopy Techniques
- Nanotechnology
Background:
- Traditional scanning transmission electron microscopy (STEM) often requires complex adjustments to electron optics for different imaging modes.
- Achieving high-precision specimen manipulation is crucial for advanced microscopy applications.
Discussion:
- The developed stage-scanning system integrates a piezo-driven stage, power supply, and control software within a specialized transmission electron microscopy (TEM) specimen holder.
- This integrated system facilitates three-dimensional scanning of the specimen, enabling confocal STEM imaging without altering the electron-optics configuration.
Key Insights:
- Confocal scanning transmission electron microscopy (STEM) images with lateral atomic resolution have been successfully obtained using the stage-scanning system.
- The system demonstrates precise three-dimensional movement capabilities for the specimen, crucial for detailed nanoscale analysis.
Outlook:
- This technology has the potential to advance nanoscale imaging and analysis in materials science.
- Further development could lead to more sophisticated in-situ experiments and correlative microscopy techniques.
Related Concept Videos
Confocal Fluorescence Microscopy
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

