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Isolation of mitochondria from cell cultures by PCT for proteomic analysis

Vera Gross1, Alexander Lazarev, Nathan Lawrence

  • 1Pressure BioSciences, Inc., South Easton, MA 02375, USA.

Biotechniques
|July 10, 2008
PubMed
Abstract

No abstract available in PubMed .

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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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