Amperometric glucose biosensor based on boron-doped carbon nanotubes modified electrode

Area of Science:

• Nanomaterials Science
• Electrochemistry
• Biotechnology

Background:

• Doped carbon nanotubes offer unique physicochemical properties for bioanalytical applications.
• Boron-doped carbon nanotubes (BCNTs) exhibit enhanced electrocatalytic activity compared to undoped counterparts.
• Defective sites induced by boron doping, rich in edge sites and oxygen groups, improve electrocatalysis.

Purpose of the Study:

• To develop and evaluate an amperometric biosensor using boron-doped carbon nanotubes (BCNTs).
• To investigate the electrocatalytic properties of BCNTs for hydrogen peroxide oxidation.
• To immobilize glucose oxidase (GOD) onto a BCNT-modified electrode for biosensing applications.

Main Methods:

• Modification of a glassy carbon (GC) electrode with BCNTs.
• Immobilization of glucose oxidase (GOD) using a poly(o-aminophenol) film.
• Electrochemical characterization using amperometric methods.

Main Results:

• The BCNT-modified electrode demonstrated significantly higher electrocatalytic activity for H2O2 oxidation than undoped CNTs.
• The developed GOD/BCNT/GC biosensor exhibited high sensitivity (171.2 nA mM⁻¹), a low detection limit (3.6 μM), and a rapid response time (<6s).
• The biosensor showed good stability, anti-interference ability, and applicability in whole blood samples with an apparent Michaelis-Menten constant of 15.19 mM.

Conclusions:

• BCNTs are effective electrocatalysts for hydrogen peroxide detection in biosensors.
• The GOD/BCNT/GC biosensor offers excellent performance characteristics for practical bioanalytical applications.
• Boron doping is a promising strategy to enhance the performance of carbon nanotube-based biosensors.