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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 3, 2026

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Flow cytometry applications for exotic animals.

Stephen A Kania¹

¹Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37849, USA. skania@utk.edu

The Veterinary Clinics of North America. Exotic Animal Practice

|August 5, 2008

Summary

This summary is machine-generated.

This article explains flow cytometry applications in hematology, detailing cell enumeration and function measurement. It also addresses reagent limitations and antibody cross-reactivity challenges in exotic animal research.

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Area of Science:

Hematology
Immunology
Cell Biology

Background:

Flow cytometry is a powerful technique for analyzing cells.
Its applications in hematology are diverse, ranging from diagnostics to research.

Purpose of the Study:

To describe the fundamental principles of flow cytometry.
To outline its applications in hematology, including cell enumeration and functional assays.
To discuss challenges related to reagent availability and antibody cross-reactivity, particularly in non-model organisms.

Main Methods:

Basic principles of flow cytometer operation.
Enumeration of cell populations using phenotypic markers.
Measurement of cellular functions via flow cytometry.

Main Results:

Flow cytometry enables precise quantification of cell populations.
It allows for the assessment of various cell functions.
Limitations in reagents and antibody cross-reactivity pose challenges for exotic animal studies.

Conclusions:

Flow cytometry is a versatile tool in hematology.
Understanding its applications and limitations is crucial for accurate analysis.
Further development of reagents is needed for broader application in comparative hematology.