Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated mapping of DNA replication fork progression in human cells with ForkML.

Nature communications·2026
Same author

Replication program of a single-chromosome budding yeast strain.

Nucleic acids research·2025
Same author

The double life of mammalian DNA replication origins.

Genes & development·2025
Same author

Dual DNA replication modes: varying fork speeds and initiation rates within the spatial replication program in Xenopus.

Nucleic acids research·2025
Same author

Telomere-to-telomere DNA replication timing profiling using single-molecule sequencing with Nanotiming.

Nature communications·2025
Same author

The next-generation sequencing-chess problem.

NAR genomics and bioinformatics·2024

Related Experiment Video

Updated: Jul 3, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

A dynamic stochastic model for DNA replication initiation in early embryos.

Arach Goldar1, Hélène Labit, Kathrin Marheineke

  • 1Service de Biologie Intégrative et de Génétique Moléculaire, Commissariat à l'Energie Atomique, Gif-sur-Yvette, France. arach.goldar@cea.fr

Plos One
|August 7, 2008
PubMed
Summary

Early embryonic cells use a novel model combining factor availability and origin affinity to regulate DNA replication initiation. This ensures timely replication completion despite stochastic origin activation, crucial for cell division.

More Related Videos

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Related Experiment Videos

Last Updated: Jul 3, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Eukaryotic cells must ensure timely DNA replication completion despite stochastic origin activation.
  • Early Xenopus embryos face challenges in replication timing due to random origin usage.
  • The molecular mechanism driving the increase in initiation rate during S phase was previously unknown.

Purpose of the Study:

  • To refine the temporal profile of DNA replication initiation rates.
  • To develop a robust model explaining replication origin usage in early embryonic S phase.
  • To investigate the molecular mechanisms underlying the regulation of initiation rate.

Main Methods:

  • Utilized DNA combing and kinetic modeling with Xenopus egg extracts.
  • Performed numerical simulations and compared them with experimental DNA combing data.
  • Tested and compared several biochemical models for replication initiation regulation.

Main Results:

  • Confirmed that the initiation rate (I(t)) increases through S phase and then decreases before completion.
  • Found that simple models (e.g., component recycling, increased origin efficiency) could not fully explain the data.
  • Developed and validated a novel model combining time-dependent factor availability and origin affinity.

Conclusions:

  • Presented a refined temporal profile of replication initiation rates during early embryonic S phase.
  • Established a robust dynamic model that quantitatively explains replication origin usage.
  • Highlighted the implications of these findings for understanding replication origin organization in higher eukaryotes.