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Updated: Jul 2, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Particle size characterization by quadruple-detector hydrodynamic chromatography.

Amandaa K Brewer¹, André M Striegel

¹Department of Chemistry & Biochemistry, Florida State University, Tallahassee, FL, 32306-4390, USA.

Analytical and Bioanalytical Chemistry

|September 3, 2008

Summary

This study introduces a novel quadruple-detector hydrodynamic chromatography method for precise particle size and shape analysis. The technique accurately characterizes diverse particles, including solid and hollow latexes, advancing material property understanding.

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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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Published on: September 11, 2020

Area of Science:

Materials Science
Analytical Chemistry
Polymer Science

Background:

Particle size and shape distribution critically impact material properties in powders, solutions, and suspensions.
Existing particle characterization methods often struggle with polydisperse or nonspherical particles.

Purpose of the Study:

To develop and apply a multi-detector hydrodynamic chromatography (HDC) method for comprehensive particle characterization.
To analyze the size, shape, and structure of polystyrene latexes ranging from 40-400 nm.

Main Methods:

Utilized quadruple-detector HDC coupled with static multiangle light scattering (MALS), quasi-elastic light scattering (QELS), differential viscometry (VISC), and differential refractometry (DRI).
Applied self-similarity scaling relationships to determine molar mass, particle radii, and shape characteristics.
Employed the dimensionless ratio rho (R(G,z)/R(H,z)) to confirm particle shape and structure.

Main Results:

Successfully determined multiple size parameters, polydispersity, and size distribution across elution profiles for polystyrene latexes.
Ascertained latex shape and shape constancy as a function of particle size using scaling relationships.
Differentiated between solid and hollow polystyrene latex samples and provided insights into their structure.

Conclusions:

The developed HDC/MALS/QELS/VISC/DRI method offers robust characterization of particle size, shape, and structure.
This technique is effective for analyzing both solid and hollow latexes and holds potential for nonspherical and fractal objects.