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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

Instrument Calibration

Instrument Calibration

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
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Standardization, calibration, and control in flow cytometry.

Robert A Hoffman¹

¹BD Biosciences, San Jose, California, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

This study details how particles are crucial for calibrating flow cytometers. It provides essential background, methods, and practical advice for instrument standardization using particles.

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Area of Science:

Biotechnology
Analytical Chemistry

Background:

Flow cytometers measure particle characteristics.
Particles are essential for instrument calibration and standardization.

Purpose of the Study:

To define critical terms related to flow cytometry calibration.
To provide practical methods for standardizing flow cytometers using particles.

Main Methods:

Review of particle types and associated cautions.
Description of practical standardization techniques for optical alignment, fluorescence, and light scatter.
Analysis of particles for calibration purposes.

Main Results:

Clarification of terminology for instrument calibration.
Detailed procedures for standardizing flow cytometer performance.
Guidance on selecting and analyzing calibration particles.

Conclusions:

Standardized particle analysis is key to reliable flow cytometry.
Understanding particle properties ensures accurate instrument calibration.
This work offers a practical framework for flow cytometer standardization.