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Synthesis of a photoresponsive alpha-dideoxyuridine triphosphate derivative
Yukari Nitta1, Masayuki Ogino, Yoshinaga Yoshimura
1SC World Inc., 529, Takata, Toyama, 930-0866, Japan.
Abstract:
The synthesis of a photoresponsive alpha-5-cyanovinyl- 2',3'-dideoxyuridine-5'-triphosphate (alpha dd(C)UTP) is described. A one-pot enzymatic synthesis of photoresponsive alpha-5-cyanovinyl-2',3'-dideoxyuridine (alpha dd(C)U) labeled ODNs with terminal deoxynucleotideyl transferase (TdT) would be a powerful tool for the development of modified long-chain DNA and branched DNA unable to be prepared by DNA synthesizer or enzymatic ligation.
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