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Updated: Dec 15, 2025

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy
Published on: September 29, 2016
Structural basis of the signal transduction in the two-component system
Seiji Yamada1, Yoshitsugu Shiro
1Biometal Science Laboratory, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
Abstract:
Two-component system (TCS) consists of two multi-domain proteins, a sensor histidine kinase (HK) and a response regulator (RR). In response to environmental change, the signal is transduced from HK to RR through phosphoryl transfer. At the first stage of structural biology of TCS, crystallographic and NMR analyses of domain blocks revealed the folds and the remarkable regions of sensor, dimerization and catalytic domains of HK and receiver and effecter domains of RR. As the second stage, the advanced researches of their multi-domain form and HK/RR complex showed the inter-domain and inter-molecular interactions and implied that the dynamic conformation changes are required in the signaling process. Thus, this chapter describes what these structural analyses of TCS proteins have contributed in understanding the cell signaling mechanism; signal input --> phosphoryl transfer --> signal output.
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