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[Effect of HBV X gene on mitochondria in HL-7702 cells]
Na Lin1, Dan Li, Hong-Ying Chen
1Department of Gastroenterology, Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Aim:
To study the effect of HBx on a new HBx-interacted protein COXIII and mitochondria.
Methods:
A recombinant eukaryotic expression plasmid pcDNA3-X was constructed, then pcDNA3-X and pcDNA3 were steadily transfected in HL-7702 separately, named HL-7702/HBx and HL-7702/pcDNA(3). The expression of COXIII in HL-7702/HBx, HL-7702/pcDNA3 and HL-7702 cells were detected by RT-PCR and western blot. Mitochondrial cytochrome c oxidase activities of three groups were assayed spectrophotometrically at 550 nm by using partially purified mitochondria.
Results:
COXIII mRNA expression didn't change, while protein expression is down-regulated in HL-7702/HBx. Cytochrome c oxidase activity was much lower in HL-7702/HBx compared with HL-7702 and HL-7702/pcDNA3 cells.
Conclusion:
HBx can down-regulate COXIII expression through post-transcriptional level and inhibit mitochondrial cytochrome c oxidase activity.
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