Measuring left ventricular function in the normal, infarcted and CORM-3-preconditioned mouse heart using complex...

Area of Science:

• Cardiovascular Research
• Physiology
• Biomedical Engineering

Background:

• Miniaturized conductance catheters are used to assess left ventricular (LV) function in mice for cardiac interventions.
• Complex admittance offers real-time measurement correction, unlike traditional conductance catheters.
• Validating complex admittance for LV function assessment in normal and infarcted hearts is crucial.

Purpose of the Study:

• To validate the ADVantage system, a complex admittance-based method, for assessing LV function.
• To compare the ADVantage system with traditional conductance-derived pressure-volume (PV) systems.
• To evaluate the system's ability to detect LV dysfunction in a mouse model of myocardial infarction.

Main Methods:

• Direct comparison of the ADVantage system and a traditional PV system using a single conductance catheter in mice.
• Induction of focal myocardial ischemia-reperfusion injury to create dysfunctional LV.
• Measurement of cardiac function to differentiate normal from dysfunctional LV contractile performance.

Main Results:

• Complex admittance provides reproducible and reliable LV pressure-volume loops, comparable to conductance catheters.
• The ADVantage system recorded larger systolic LV cavity volumes than the traditional system.
• Significant LV dysfunction was observed post-coronary artery occlusion, ameliorated by CORM-3 (carbon monoxide-releasing molecule).

Conclusions:

• The ADVantage system effectively and reproducibly measures LV function and dysfunction in mice.
• This system eliminates the need for complex calibration interventions.
• The ADVantage system shows promise for accurate murine cardiac function assessment.