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Updated: Jun 27, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
[Terpenoids from leaves of Aeschynanthus mengxinensis]
Wen-yi Kang1, Xin-yan Zang, Jin-mei Wang
1Institute of Natural Products, Henan University, Kaifeng 475004, China. kangweny@hotmail.com
Objective:
To study the chemical constituents from the ethyl acetate portion of an ethanolic extractive of the leaves of Aeschynanthus mengxinensis.
Method:
The column chromatographic techniques were applied to isolate constituents. A combination of IR, ESI-MS, NMR and 2D-NMR spectroscopy was used to identify structures.
Result:
Four compounds were isolated from the ethyl acetate fraction of this plant, and the structure of them have identified as 2alpha, 3beta, 19beta-trihydroxyolean-12-ene-23,28-dioic acid (1), 2alpha, 3beta, 21beta-trihydroxyolean-12-ene-28-oic acid (2), 2alpha, 3beta, 23-trihydroxyurs-12-ene-28-oic acid (3) and stigmast-5 (6), 22 (23)-diene-3beta-ol (4).
Conclusion:
The NMR data of compound 1 was completely assigned by 2D-NMR techniques, including HMBC and HMQC. Compounds 1-4 were isolated for the first time from Gesneriaceae.
