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Updated: Jun 27, 2026

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
Published on: October 4, 2013
Negative feedback that improves information transmission in yeast signalling.
Richard C Yu1, C Gustavo Pesce, Alejandro Colman-Lerner
1Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, California 94704, USA. ryu@molsci.org
Yeast cells use negative feedback to improve signal transmission during mating. This mechanism aligns cellular responses to pheromone concentration, enhancing information fidelity and reducing noise.
Area of Science:
- Cellular signaling
- Molecular biology
- Yeast genetics
Background:
- Haploid yeast cells (Saccharomyces cerevisiae) possess a cell signaling system to detect mating pheromones.
- Effective response to varying pheromone concentrations is crucial for mating and depends on information transmission fidelity.
Purpose of the Study:
- To investigate the role of negative feedback in the yeast mating pheromone response pathway.
- To understand how dose-response alignment impacts information transmission fidelity.
Main Methods:
- Investigated the function of the mitogen-activated protein kinase Fus3 in negative feedback.
- Analyzed the impact of Fus3-mediated feedback on downstream signaling pathways.
- Identified Sst2 as a target of this negative feedback loop.
Main Results:
- Fus3 mediates fast-acting negative feedback, aligning the dose response of the system to receptor-ligand binding.
- This 'dose-response alignment' creates a linear relationship between receptor occupancy and downstream response.
- Discovered a signal-promoting function of Sst2, a regulator of G-protein signaling, as a target of this feedback.
Conclusions:
- Negative feedback is a key mechanism for achieving dose-response alignment in signaling systems.
- Dose-response alignment enhances information transmission fidelity by improving distinguishability of responses and reducing noise amplification.
- This study reveals a novel role for Sst2 and highlights negative feedback as a general strategy for robust signaling.
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