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Absolute brightness of fluorescent microspheres
Isaac Finger1, Scott Phillips, Elizabeth Mobley
1Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611-6400, USA.
Lab on a Chip
|January 22, 2009
Summary
The absolute brightness of fluorescent microspheres was measured, providing key data for developing advanced fluorescence detection technologies and smart dust devices.
Area of Science:
- Nanotechnology and Materials Science
- Biophysics
Background:
- Absolute brightness is crucial for fluorescence detection applications.
- Fluorescent particles like microspheres and quantum dots are widely used.
Purpose of the Study:
- To measure the absolute brightness of nile-red fluorescent microspheres.
- To discuss the implications for designing smart dust devices and remote fluorescence detection.
Main Methods:
- Measurement of absolute brightness for 1-micrometer diameter nile-red fluorescent microspheres.
Main Results:
- The absolute brightness was determined to be 4.2 +/- 1 x 10(-16) m(2)/sr.
Conclusions:
- The measured absolute brightness is a critical parameter for fluorescence-based applications.
- Findings inform the development of kinesin motor protein-powered smart dust and remote fluorescence sensing.
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