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[Apoptosis of laryngeal carcinoma cells induced by antisense oligodeoxynucleotides complementary Stat3 in vitro]
Haili Lü1, Qiuhang Zhang, Bo Yan
1Department of Otolaryngology-Head and Neck Surgery, Xuanwu Hospital, Capital University of Medical Science, Beijing, 100053, China. lvhaili@hotmail.com
Objective:
To study the effects of oligodeoxynucleotides complementary Stat3 on apoptosis in laryngeal carcinoma Hep-2 cell.
Method:
Oligodeoxynucleotides complementary Stat3 was designed, which was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Expression of Stat3 and p-Stat3 were detected by Western blot and PCR. MTT was used to observe the growth-inhibiting ratio. DNA ladder, AO/EB and FCM were used to observe the apoptosis of laryngeal carcinoma cell Hep-2 in vitro.
Result:
Western blot and PCR results demonstrated that oligodeoxynucleotides complementary Stat3 could significantly inhibit the expression of Stat3 and p-Stat3 in Hep-2 cell. MTT results showed that it could significantly suppress the growth of Hep-2 cell. The DNA ladder, AO/EB and FCM results showed it could inhibit the expression of Stat3 and induce the apoptosis of Hep-2 cell in a concentration-dependent manner.
Conclusion:
Oligodeoxynucleotides complementary Stat3 could induce the apoptosis and suppress cell proliferation in laryngeal carcinoma Hep-2 cell.
Insights
Oligodeoxynucleotides targeting Signal Transducer and Activator of Transcription 3 (Stat3) effectively induce apoptosis and inhibit proliferation in laryngeal carcinoma Hep-2 cells.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Context:
- Laryngeal carcinoma is a significant global health concern.
- Signal Transducer and Activator of Transcription 3 (Stat3) is implicated in various cancers, including laryngeal carcinoma.
- Targeting Stat3 signaling is a potential therapeutic strategy.
Purpose:
- To investigate the effect of oligodeoxynucleotides complementary to Stat3 on apoptosis in laryngeal carcinoma Hep-2 cells.
- To assess the impact of Stat3 inhibition on cell growth and apoptosis.
- To evaluate the expression levels of Stat3 and its phosphorylated form (p-Stat3).
Summary:
- Oligodeoxynucleotides complementary to Stat3 were designed and delivered into Hep-2 cells via lipofection.
- Western blot and PCR confirmed significant inhibition of Stat3 and p-Stat3 expression.
- MTT assays demonstrated suppressed cell growth, while DNA ladder, AO/EB staining, and FCM analysis revealed concentration-dependent induction of apoptosis.
Impact:
- This study demonstrates that Stat3 inhibition using complementary oligodeoxynucleotides can effectively induce apoptosis and suppress proliferation in laryngeal carcinoma cells.
- Findings suggest a potential therapeutic role for Stat3-targeting oligodeoxynucleotides in treating laryngeal cancer.
- Provides a foundation for further research into Stat3-based therapies for head and neck cancers.
