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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
High-resolution surface plasmon resonance sensors based on a dove prism
Olivier R Bolduc1, Ludovic S Live, Jean-François Masson
1Département de Chimie, Université de Montréal, C.P. 6128 Succ. Centre-Ville, Montréal, Qc, Canada H3C 3J7.
Talanta
|January 23, 2009
Summary
A novel Surface Plasmon Resonance (SPR) biosensor utilizes a dove prism for high-resolution refractive index monitoring and imaging. This cost-effective system enhances sensitivity and enables detection of low-concentration biological events.
Area of Science:
- Optical Spectroscopy
- Biosensing Technology
- Surface Plasmon Resonance (SPR)
Background:
- Surface Plasmon Resonance (SPR) spectroscopy is a powerful technique for real-time monitoring of molecular interactions.
- Traditional SPR systems can be complex and expensive, limiting their widespread application.
- There is a need for simplified, cost-effective SPR instruments with high analytical performance.
Purpose of the Study:
- To develop and characterize a novel SPR biosensor system using a dove prism for wavelength interrogation.
- To evaluate the system's performance in terms of refractive index (RI) sensitivity, resolution, and reproducibility.
- To demonstrate the system's capability for biosensing and SPR imaging of low-concentration biological events.
Main Methods:
- A BK7 dove prism was integrated into an SPR setup for wavelength interrogation, enabling image inversion and utilizing a total internal reflection angle of 72.8 degrees.
- Fluidics were incorporated for efficient sample delivery.
- Data analysis methodologies, including minimum hunting and singular value decomposition (SVD), were employed to enhance resolution.
Main Results:
- The SPR system demonstrated high refractive index (RI) sensitivity and resolution in the 10(-6) range, improved by one order of magnitude with SVD data pre-treatment.
- Tunable RI ranges of 1.32-1.39 RIU and 1.32-1.42 RIU were achieved using different spectrophotometers.
- The system successfully monitored microM concentrations of beta-lactamase and obtained SPR images of a 500 nL water droplet.
Conclusions:
- The dove prism SPR configuration offers a unique platform combining advantages of portable, imaging, and research-grade SPR instruments.
- This simplified optical design provides high analytical performance for refractive index monitoring and biosensing applications.
- The developed SPR biosensor is suitable for detecting low-concentration biological events and performing SPR imaging.
