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Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice
Published on: March 30, 2022
Increased myocyte content and mechanical function within a tissue-engineered myocardial patch following implantation.
Damon J Kelly1, Amy B Rosen, Adam J T Schuldt
11 Institute for Molecular Cardiology, Stony Brook University , Stony Brook, New York, USA.
Tissue Engineering. Part A
|February 24, 2009
Summary
Extracellular matrix (ECM) scaffolds enhance heart repair by increasing myocyte content and improving regional function over time. This study quantifies cell populations, revealing a direct correlation between myocyte quantity and mechanical performance in myocardial defects.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Cardiovascular Research
Background:
- Studies show stem cells, chemical factors, and scaffolds can augment the mammalian heart's regenerative capacity.
- Extracellular matrix (ECM) scaffolds are used for myocardial defect repair, becoming populated with myocytes and providing contractile function.
- Quantification of myocyte populations within ECM scaffolds and their correlation with mechanical function has not been previously conducted.
Purpose of the Study:
- To quantitate myocyte content within xenogenic ECM bioscaffolds.
- To correlate myocyte population with regional mechanical function over time.
- To assess the efficacy of ECM scaffolds in repairing myocardial defects.
Main Methods:
- Xenogenic ECM scaffolds from porcine urinary bladder were implanted into surgically induced right ventricular-free wall defects in a dog model.
- Regional function and myocyte content were determined at 0, 2, and 8 weeks post-implantation.
- Quantitative analysis of myocyte content and regional mechanical function (stroke work, systolic contraction) was performed.
Main Results:
- Regional function did not significantly increase between 0 and 2 weeks post-implantation.
- By 8 weeks, regional stroke work increased to 3.7 +/- 0.7% and systolic contraction to 4.4 +/- 1.2%.
- A significant increase in myocyte content was observed, demonstrating a linear relationship with improved regional function.
Conclusions:
- ECM scaffolds, when used as myocardial patches, promote increased regional function and myocyte content over time.
- The mechanical function achieved in the repaired region is directly correlated with the local myocyte quantity.
- ECM scaffolds represent a promising approach for enhancing cardiac regeneration and function.
