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Updated: Jun 25, 2026

High-Throughput Bioprinting Method for Modeling Vascular Permeability in Standard Six-well Plates with Size and Pattern Flexibility
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High-Throughput Bioprinting Method for Modeling Vascular Permeability in Standard Six-well Plates with Size and Pattern Flexibility

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Engineering high-density endothelial cell monolayers on soft substrates.

Adam W Feinberg1, James F Schumacher, Anthony B Brennan

  • 1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, 32611-6400, USA.

Acta Biomaterialia
|March 10, 2009
PubMed
Summary
This summary is machine-generated.

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Tissue engineering of endothelial cells (ECs) achieved in vivo-like density on polydimethylsiloxane (PDMS) using microcontact printing of fibronectin (FN). This method promotes cell adhesion and growth for creating functional cell monolayers.

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cell Biology

Background:

  • Endothelial cells (ECs) form a critical barrier in vivo.
  • Replicating in vivo cell density and morphology in engineered tissues is challenging.
  • Current methods often fail to achieve sufficient EC density and confluence on artificial substrates.

Purpose of the Study:

  • To develop a method for tissue engineering endothelial cell monolayers with in vivo-like cell density and morphology.
  • To investigate the effect of microscale fibronectin patterning on EC adhesion, growth, and density.
  • To compare EC behavior on patterned versus uniformly coated substrates.

Main Methods:

  • Microcontact printing of fibronectin (FN) in a square lattice array on polydimethylsiloxane (PDMS) elastomer.

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Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

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Last Updated: Jun 25, 2026

High-Throughput Bioprinting Method for Modeling Vascular Permeability in Standard Six-well Plates with Size and Pattern Flexibility
07:41

High-Throughput Bioprinting Method for Modeling Vascular Permeability in Standard Six-well Plates with Size and Pattern Flexibility

Published on: August 16, 2024

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

  • Culture of endothelial cells on micropatterned PDMS and control surfaces.
  • Quantification of cell density, confluence, and focal adhesion formation.

    • Main Results:

    • A confluent EC monolayer was achieved on FN-micropatterned PDMS with a cell density of 1,536+/-247 cells/mm², approximating in vivo conditions.
    • Uniform FN or serum protein coatings failed to support equivalent EC density or confluence.
    • Micropatterning significantly increased focal adhesion formation (35+/-10 FAs/cell vs. 7+/-6 FAs/cell).

    Conclusions:

    • Microscale patterning of fibronectin on PDMS elastomer promotes the formation of endothelial cell monolayers with in vivo-like cell density and morphology.
    • The enhanced EC adhesion, growth, and density are attributed to increased focal adhesion formation.
    • This technique offers a promising approach for engineering functional endothelialized surfaces.