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Updated: Jun 24, 2026
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
Comparison of two methods for the quantification and identification of hemoglobin variants
Trefor Higgins1, Melissa Mack, Annu Khajuria
1DynaLIFEDx, Chemistry Department, Edmonton, Alberta, Canada. trefor.higgins@dynalifedx.com
Background:
The Sebia Capillarys 2, a capillary electrophoresis method, was compared to a high performance liquid chromatography (HPLC) and electrophoresis at alkaline and acid pH for the presumptive identification and quantitation of hemoglobin variants.
Methods:
The concordance of hemoglobin variant identification on the Sebia Capillarys 2 with the combination of HPLC and electrophoresis at alkaline and acid pH was evaluated by analyzing samples on both systems. The quantification, expressed as % of total hemoglobin, of the common hemoglobin variants on the Capillarys 2 and the Bio Rad VARIANT II beta thalassemia methods were compared.
Results:
The % hemoglobin variant results for the two methods were similar for HbS and slightly different for HbC and D Punjab and significantly different for HbE. The Sebia Capillarys 2 correctly identified a number of hemoglobin variants.
Conclusion:
The Sebia Capillarys 2 is suitable for the presumptive identification and quantification of hemoglobin variants producing results comparable with existing HPLC and electrophoresis methods.
