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Updated: Jun 24, 2026
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Published on: April 3, 2017
[Expression profile of ADRP in macrophages and foam cells]
Yue Song1, Hang Li, Li-ying Zhang
1Department of Pathology and Pathophysiology, the Fourth Military Medical University, Xi'an 710032, China. liqing@fmmu.edu.cn
Aim:
To investigate the expression profile of adipose differentiation-related protein(ADRP)in macrophages and foam cells.
Methods:
THP-1 monocytes were differentiated into macrophages in vitro, and then induced into foam cells by culturing with oxidized low density lipoproteins(ox-LDLs). Real time RT-PCR and Western blot were performed to detect the expression of ADRP gene and protein.
Results:
The expression of ADRP both mRNA and protein were significantly increased when macrophages were further induced into foam cells by ox-LDLs, however, the same results were not observed during phorbol 12-myristate-13-acetate(PMA)-induced monocyte differentiation into macrophages.
Conclusion:
Our findings revealed an upregulation of ADRP during macrophages differentiation into foam cells.
