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Updated: Jun 24, 2026

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model
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Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

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Human bladder cancer diagnosis using Multiphoton microscopy.

Sushmita Mukherjee1, James S Wysock, Casey K Ng

  • 1Department of Biochemistry, Weill Cornell Medical College, New York, NY.

Proceedings of Spie--The International Society for Optical Engineering
|April 11, 2009
PubMed
Summary
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Multiphoton imaging accurately identifies non-muscle invasive bladder cancers in fresh biopsies. This technique shows promise for improving early bladder cancer diagnosis and surgical margin assessment, potentially reducing recurrence rates.

Area of Science:

  • Biomedical Optics
  • Uropathology
  • Cancer Imaging

Background:

  • Non-muscle invasive bladder cancer (NMIBC) accounts for ~75% of diagnoses.
  • Conventional cystoscopy often misses small or flat NMIBC lesions, leading to high recurrence rates.
  • Current follow-up involves repeated costly and morbid cystoscopies and biopsies.

Purpose of the Study:

  • To evaluate Multiphoton imaging for real-time, non-invasive diagnosis of NMIBC in human bladder biopsies.
  • To assess Multiphoton imaging's potential for evaluating surgical resection margins.

Main Methods:

  • Multiphoton microscopy was used on fresh, unfixed human bladder biopsies.
  • Imaging utilized broadband autofluorescence and second harmonic generation (SHG) channels.
  • Multiphoton images were compared to gold standard hematoxylin/eosin (H&E) stained histopathology.

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A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
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Last Updated: Jun 24, 2026

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model
06:12

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

Published on: May 29, 2015

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
06:23

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

Published on: January 12, 2017

Main Results:

  • Multiphoton imaging demonstrated excellent correlation with histopathological diagnoses in training and blinded sample sets.
  • The technique effectively identified cancerous tissue in fresh bladder biopsies.
  • Ongoing larger blinded analysis with uropathologists aims to quantify diagnostic accuracy.

Conclusions:

  • Multiphoton imaging shows significant potential for accurate, non-invasive diagnosis of NMIBC.
  • This technology could improve early detection and surgical assessment, reducing the need for repeat procedures.
  • Further validation is underway to establish diagnostic accuracy and inter-observer reliability.