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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jun 24, 2026

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Common-path multimodal optical microscopy.

Chandra S Yelleswarapu1, Marla Tipping, Sri-Rajasekhar Kothapalli

  • 1Department of Physics, University of Massachusetts, 100 Morrissey Boulevard, Boston, Massachusetts 02125, USA.

Optics Letters
|April 17, 2009
PubMed
Summary
This summary is machine-generated.

A new common-path multimodal optical microscopy system images amplitude, phase, and fluorescence from a single source and camera. This advanced microscopy provides structural and functional data for live-cell dynamics and automated microscopy applications.

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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

  • Optical microscopy
  • Biomedical imaging
  • Cell biology

Background:

  • Multimodal imaging offers comprehensive biological insights.
  • Integrating multiple imaging modalities can be complex and require sample manipulation.
  • Existing systems often necessitate image registration and fusion for multimodal analysis.

Purpose of the Study:

  • To develop a simplified common-path multimodal optical microscopy system.
  • To enable simultaneous imaging of amplitude, phase, and fluorescence from a single setup.
  • To demonstrate the system's capability without image registration.

Main Methods:

  • Utilized a single optical source and a single camera.
  • Employed Fourier plane contrast enhancement filters and filters in front of the CCD camera.
  • Varied filter configurations to acquire different imaging modalities.

Main Results:

  • Successfully acquired brightfield, fluorescence, phase-contrast, and combined images of a Drosophila embryo.
  • Demonstrated simultaneous imaging of structural and functional features.
  • Achieved multimodal imaging without the need for image registration and fusion.

Conclusions:

  • The developed system provides a versatile platform for multimodal optical microscopy.
  • This approach simplifies the acquisition of comprehensive biological data.
  • Potential applications include live-cell dynamics, high-throughput, and automated microscopy.