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Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Updated: Jun 23, 2026

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Published on: June 13, 2018

Shine a light: imaging the immune system.

Milka Sarris¹, Alexander G Betz

¹Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK. betz@mrc-lmb.cam.ac.uk

European Journal of Immunology

|May 1, 2009

Summary

This summary is machine-generated.

Intra vital microscopy and whole-body imaging offer new ways to study the immune system. Combining these advanced imaging techniques with traditional methods will provide a comprehensive understanding of immune processes.

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Area of Science:

Immunology
Microscopy
In vivo imaging

Background:

Intra vital microscopy and whole-body imaging are revolutionizing immune system research.
These techniques provide direct biological relevance and visually compelling data.
Ex vivo reductionist approaches have historically yielded fundamental insights.

Purpose of the Study:

To provide an overview of available imaging techniques for studying the immune system.
To discuss the strengths and limitations of various imaging modalities.
To highlight how these tools have advanced current understanding and to propose future directions.

Main Methods:

Review of intra vital microscopy techniques.
Analysis of whole-body imaging approaches.
Discussion of ex vivo methods, including microfluidics.

Main Results:

Imaging techniques offer direct visualization of immune processes.
Both in vivo and ex vivo methods have contributed significantly to the field.
Microfluidics presents new opportunities for ex vivo immune studies.

Conclusions:

Integrating findings from diverse imaging disciplines is crucial.
The future requires applying the most suitable technologies for a unified view of the immune system.
A comprehensive "big picture" of immune system function is the ultimate goal.