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Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
Published on: October 17, 2016
Re-evaluation of model-based light-scattering spectroscopy for tissue spectroscopy
Condon Lau1, Obrad Sćepanović, Jelena Mirkovic
1Massachusetts Institute of Technology, George R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Journal of Biomedical Optics
|May 2, 2009
Summary
Model-based light scattering spectroscopy (LSS) for dysplasia diagnosis is flawed. The residual spectrum is mainly due to hemoglobin distribution in blood vessels, not nuclear scattering, necessitating revised models.
Area of Science:
- Biomedical Optics
- Medical Diagnostics
- Spectroscopy
Background:
- Model-based light scattering spectroscopy (LSS) was considered promising for in-vivo diagnosis of dysplasia.
- Existing models attributed the residual spectrum to nuclear light scattering, extracting diagnostic information from it.
Purpose of the Study:
- To re-evaluate the source of the residual spectrum in model-based LSS.
- To investigate the impact of hemoglobin distribution on diffuse reflectance measurements.
Main Methods:
- Numerical simulations based on light propagation and Mie theory.
- Tissue phantom experiments.
- Analysis of published spectroscopic data from Barrett's esophagus.
Main Results:
- The residual spectrum is not primarily from nuclear scattering but largely from assuming uniform hemoglobin distribution.
- Hemoglobin packaging in blood vessels significantly alters tissue reflectance.
- Including vessel packaging in diffuse reflectance models improves accuracy and reduces the residual spectrum.
Conclusions:
- The current interpretation of residual spectra in model-based LSS is incorrect.
- Vessel packaging must be incorporated into diffuse reflectance models for accurate tissue analysis.
- The use of current model-based LSS for dysplasia diagnosis should be discontinued.
