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Updated: Jun 23, 2026
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Bioelectrochemical sensing based on single stranded deoxyribonucleic acid-carbon nanotubes covalently attached on
Lenibel Santiago-Rodríguez1, Nella M Vargas-Barbosa, Germarie Sánchez-Pomales
1Department of Chemistry and Center for Nanoscale Materials, University of Puerto Rico, Rio Piedras Campus, P.O. Box 23346, San Juan, Puerto Rico 00931-3346.
Abstract:
A biosensor based on single-stranded deoxyribonucleic acid-functionalized carbon nanotubes covalently attached to a self-assembled monolayer of 11-amino-1-undecanethiol on gold has been prepared. The preparation of the deoxyribonucleic acid sensor was followed using cyclic voltammetry. Single-walled carbon nanotubes, covalently attached to the gold surface present a nanoelectrode array behavior. To confirm the hybridization step between the probe and the target deoxyribonucleic acid, we used methylene blue, which is an indicator capable of distinguishing between single-stranded deoxyribonucleic acid and double-stranded deoxyribonucleic acid. Our results demonstrate that the modification of gold with single-walled carbon nanotubes and single-stranded deoxyribonucleic acid can be used for deoxyribonucleic acid hybridization detection.
