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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Fluorometric determination of nitrite with 2,3-diaminonaphthalene by reverse phase HPLC under alkaline conditions

Yang-Il Fang1, Hisayuki Ohata, Kazuo Honda

  • 1Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan. fang@pharm.showa-u.ac.jp

Journal of Pharmacological and Toxicological Methods
|June 9, 2009
PubMed
Abstract

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The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by water loss...
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