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Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
Published on: November 2, 2013
Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics
Andreas Lenshof1, Asilah Ahmad-Tajudin, Kerstin Järås
1Department of Electrical Measurements, Lund University, Box 118, 221 00 Lund, Sweden. andreas.lenshof@elmat.lth.se
Analytical Chemistry
|July 15, 2009
Summary
This study introduces an acoustophoresis chip for preparing diagnostic plasma from whole blood. The device yields high-quality plasma suitable for clinical diagnostics, including prostate-specific antigen detection.
Area of Science:
- Biomedical Engineering
- Clinical Diagnostics
- Microfluidics
Background:
- High-quality plasma generation is crucial for biomedical analyses.
- Processing whole blood in microfluidic devices presents challenges due to high cellular content.
Purpose of the Study:
- To develop and validate an acoustophoresis-based microfluidic chip for preparing diagnostic plasma from whole blood.
- To integrate the plasma separation chip with a prostate-specific antigen (PSA) detection microarray for clinical application.
Main Methods:
- Utilized acoustophoresis for sequential removal of enriched blood cells from whole blood.
- Developed a microfluidic plasmapheresis chip capable of producing low-cellular-content plasma.
- Integrated the plasma chip with a porous silicon antibody microarray for PSA detection.
Main Results:
- The acoustophoresis chip successfully generated plasma meeting clinical standards (<6.0 x 10(9) erythrocytes/L).
- Prostate-specific antigen (PSA) was detected with high linearity (R(2) > 0.99) in the processed plasma.
- Accurate PSA quantification was achieved at clinically relevant concentrations (0.19-21.8 ng/mL) without signal amplification.
Conclusions:
- Acoustophoresis is an effective method for high-quality plasma preparation from whole blood using microfluidic devices.
- The integrated system demonstrates a viable approach for point-of-care diagnostics, enabling sensitive PSA detection in processed plasma.
