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Crystallization and preliminary crystallographic study of glucose dehydrogenase from the archaebacterium Thermoplasma
J R Bright1, R Mackness, M J Danson
1Department of Biochemistry, University of Bath Claverton Down, U.K.
Journal of Molecular Biology
|November 20, 1991
Abstract:
Single crystals of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapour diffusion method and polyethylene glycol as a precipitant in the presence of NADP+ at pH 5.4. The crystals belong to the hexagonal space group P6122 or P6522, with unit cell dimensions a = b = 121.9 angstrom, c = 229.6 angstrom and with two molecules in the asymmetric unit.