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Published on: February 5, 2018
[Extraction and purification of Atractylodes macrocephala monosaccharide composition]
Zhong-Feng Shi1, Ru-Liu Li, Wei-Wen Chen
1Piwei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Summary
Water extraction followed by D900 ion exchange resin purification is an effective method for Atractylodes macrocephala monosaccharide composition (AMMC). Hydrochloric acid-ultraviolet spectrophotometry is ideal for AMMC quantitative analysis.
Area of Science:
- Phytochemistry
- Analytical Chemistry
Context:
- Atractylodes macrocephala is a traditional Chinese medicine with various bioactive compounds.
- Understanding the monosaccharide composition is crucial for its medicinal applications.
Purpose:
- To optimize the extraction and purification methods for Atractylodes macrocephala monosaccharide composition (AMMC).
- To establish a reliable analytical method for quantifying AMMC.
Summary:
- Water extraction followed by purification using neutro-alumina and D900 ion exchange resin yielded AMMC with high purity (above 76%).
- Hydrochloric acid-ultraviolet spectrophotometry proved effective for quantitative analysis of AMMC.
Impact:
- Provides an optimized and efficient method for AMMC extraction and purification.
- Establishes a validated analytical technique for AMMC quantification, supporting quality control and further research.
